EVALUATION OF DIFFERENT TECHNIQUES FOR DNA DIRECT EXTRACTION FROM BRAZILIAN AGRICULTURAL SOIL

Autores

  • Márcia Maria Rosa UNESP
  • Sâmia Maria Tauk-Tornisielo UNESP
  • Sandra Regina Ceccato-Antonini UFSCar

DOI:

https://doi.org/10.14295/holos.v10i1.1842

Palavras-chave:

Soil microbiology, DNA extraction from soil, PCR, Sugarcane

Resumo

Soil is an ecosystem characterized by a great complexity and hard to study due to its heterogeneity, especially the soil microorganism’s community. Currently, molecular biology tools have been used to study the soil biodiversity mainly through microbial genes. DNA Direct Extraction from soil is an important step in this kind of study, however the majority of techniques were developed for soils from temperate climate and just a few can be applied efficiently to Brazilian soils. This work aimed to evaluate nine different techniques for soil DNA direct extraction from sugarcane crop areas under organic and conventional managements and also to propose modifications which might result in higher DNA yield and low cost. DNA bands were observed only for three techniques (Selbach´s, Direito´s and commercial kit), two of them already tested for tropical climate soils. The best results for DNA yield (µg.g-1 soil) were obtained through Selbach´s and commercial kit techniques, however not differing statistically from those results through a protocol here proposed. This modified protocol showed the best results for DNA yield whatever soil was used. The best DNA yields were found in soil under organic management probably due to higher microbial biomass. This protocol showed better results in yield of DNA regardless of the soil used and was easier to perform and less costly.

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Publicado

2010-10-05

Como Citar

Rosa, M. M., Tauk-Tornisielo, S. M., & Ceccato-Antonini, S. R. (2010). EVALUATION OF DIFFERENT TECHNIQUES FOR DNA DIRECT EXTRACTION FROM BRAZILIAN AGRICULTURAL SOIL. Holos Environment, 10(1), 12–25. https://doi.org/10.14295/holos.v10i1.1842

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